RNA

Part:BBa_K576003:Design

Designed by: George Zarubin   Group: iGEM11_Waterloo   (2011-06-20)


Left Part of Self-Excising Ribozyme


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3


Design Notes

The P1 helix of the intron has been modified to accommodate the MetaPrefix. The part is in RFC53 L-MetaPart format, having GGT and ATG overgangs.


Source

[http://www.rna.whu.edu.cn/perl/gissd/get_seq_exon.cgi?intron_id=304 Group I intron Twort.v.ORF142,2]

References